Analysis Note

ControlHeLa cell lysate

Application

Immunoprecipitation Analysis: A representative lot was used by an independent laboratory in IP. (Kremer, B., et al. (2005). MBC. 16(10):4648-4659.)

Research Sub CategoryCell Cycle, DNA Replication & RepairCytoskeleton

Research CategoryEpigenetics & Nuclear FunctionEpigenetics & Nuclear Function

Anti-Septin-6 Antibody, clone 9E7 is a Mouse Monoclonal Antibody for the detection of Septin-6 & has been validated in WB & IP.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Septin-6 is a filament-forming GTPase that participates in cytokenisis and is necessary for the normal organization of the actin cytoskeleton. It is widely expressed in tissues and is found in the cytoplasm and in the spindle during metaphase. Four isoforms of Septin-6 have been described.
As a protein family Septins were first characterized as regulators of cytokinesis. These proteins were later shown to have a broader role in microtubule-dependent processes, such as karyokinesis, exocytosis, and maintenance of cell shape. Several septin family members are known to colocalize or interact with the microtubule cytoskeleton and are believed to play a role in regulating microtubule dynamics through interactions with microtubule-associated proteins (MAPs).

Immunogen

Epitope: Unknown

GST-tagged recombinant protein corresponding to human Septin-6.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Protein G Purified

Purified mouse monoclonal IgG3κ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.

Format: Purified

Quality

Evaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: 0.1 µg/ml of this antibody detected Septin-6 on 10 µg of HeLa cell lysate.

Specificity

Recognizes Septin-6.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~ 50 kDa